[1]方志軍.實時熒光PCR檢測乙肝病毒DNA的效果評價[J].醫學信息,2019,(22):175-176.[doi:10.3969/j.issn.1006-1959.2019.22.062]
 FANG Zhi-jun.Evaluation of the Effect of Real-time Fluorescent PCR on Detection of Hepatitis B Virus DNA[J].Medical Information,2019,(22):175-176.[doi:10.3969/j.issn.1006-1959.2019.22.062]
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實時熒光PCR檢測乙肝病毒DNA的效果評價()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2019年22期
頁碼:
175-176
欄目:
診療技術
出版日期:
2019-11-15

文章信息/Info

Title:
Evaluation of the Effect of Real-time Fluorescent PCR on Detection of Hepatitis B Virus DNA
文章編號:
1006-1959(2019)22-0175-03
作者:
方志軍12
(1.湖北省黃石市第四醫院檢驗科,湖北 黃石 435006;2.黃石醫養醫學檢驗有限公司,湖北 黃石 435006)
Author(s):
FANG Zhi-jun
(1.Department of Clinical Laboratory,the Fourth Hospital of Huangshi,Huangshi 435006,Hubei,China;2.Yiyang Medical Laboratory Of Huangshi,Huangshi 435006,Hubei,China)
關鍵詞:
實時熒光PCR檢驗乙肝病毒DNA酶聯免疫吸附檢驗法乙型肝炎
Keywords:
Real-time fluorescent PCR testHepatitis B virus DNAEnzyme-linked immunosorbent assayHepatitis B
分類號:
R446.5
DOI:
10.3969/j.issn.1006-1959.2019.22.062
文獻標志碼:
A
摘要:
目的 觀察應用實時熒光PCR檢驗法檢測乙肝病毒DNA的效果,為臨床提高診斷乙型肝炎病毒的準確性提供思路。方法 選取2015年11月~2018年7月于本院就診的458例乙型肝炎患者作為研究對象,所有患者均經標準的abbot藥盒檢測確診,其中大三陽患者241例,小三陽患者217例;颊呷朐汉缶杉崭轨o脈血,制樣本后采用酶聯免疫吸附檢驗法和實時熒光PCR檢驗,比較兩種檢驗方法對患者乙肝類型的陽性檢出率。結果 實時熒光PCR檢驗法對大三陽的陽性檢出率為95.43%,小三陽的陽性檢出率為69.58%,均高于酶聯免疫吸附檢驗法的85.06%、55.76%,差異有統計學意義(P<0.05)。結論 實時熒光PCR檢驗法可直觀的反映乙型肝炎病情狀況,具有較高的診斷效果,臨床醫師可在實際工作中根據患者經濟、病情等綜合因素進行選用。
Abstract:
Objective To observe the effect of real-time fluorescent PCR assay for detecting hepatitis B virus DNA, and to provide ideas for clinical improvement of hepatitis B virus accuracy. Methods A total of 458 patients with hepatitis B who were admitted to our hospital from November 2015 to July 2018 were enrolled in the study. All patients were diagnosed by standard abbot kit. Among them, 241 patients with Dasanyang patients and 217 patients with Xiaosanyang patients. example. Fasting venous blood was collected from all patients after admission. After the samples were prepared, enzyme-linked immunosorbent assay (ELISA) and real-time fluorescent PCR were used to compare the positive detection rates of the two test methods for patients with hepatitis B type. Results The positive detection rate of Dasanyang by real-time fluorescent PCR assay was 95.43%, and the positive detection rate of Xiaosanyang was 69.58%. Both were higher than 85.06% and 55.76% of the enzyme-linked immunosorbent assay (ELISA),the difference was statistically significant (P<0.05). Conclusion Real-time fluorescent PCR assay can directly reflect the status of hepatitis B, and has a high diagnostic effect. Clinicians can choose according to the comprehensive factors such as patient's economy and condition in actual work.

參考文獻/References:

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更新日期/Last Update: 2019-11-15
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