[1]董慶喆,李慎玲,連媛媛,等.IL-17在鼻息肉形成中的作用機制[J].醫學信息,2019,(22):59-61,65.[doi:10.3969/j.issn.1006-1959.2019.22.019]
 DONG Qing-zhe,LI Shen-ling,LIAN Yuan-yuan,et al.The Mechanism of Action of IL-17 in the Formation of Nasal Polyps[J].Medical Information,2019,(22):59-61,65.[doi:10.3969/j.issn.1006-1959.2019.22.019]
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IL-17在鼻息肉形成中的作用機制()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2019年22期
頁碼:
59-61,65
欄目:
論著
出版日期:
2019-11-15

文章信息/Info

Title:
The Mechanism of Action of IL-17 in the Formation of Nasal Polyps
文章編號:
1006-1959(2019)22-0059-04
作者:
董慶喆1李慎玲2連媛媛2徐 禛2張念凱2姜曉丹2
(青島大學附屬醫院生物標本庫1,耳鼻咽喉頭頸外科2,山東 青島 266003)
Author(s):
DONG Qing-zhe1LI Shen-ling2LIAN Yuan-yuan2XU Zhen2ZHANG Nian-kai2JIANG Xiao-dan2
(Department of Biological Specimen1,Department of Otorhinolaryngology Head and Neck Surgery2,Affiliated Hospital of Qingdao University,Qingdao 266003,Shandong,China)
關鍵詞:
鼻息肉白介素-17受體巨噬細胞
Keywords:
Nasal polypsInterleukin-17 receptorMacrophage
分類號:
R765.25
DOI:
10.3969/j.issn.1006-1959.2019.22.019
文獻標志碼:
A
摘要:
目的 檢測白介素-17(IL-17)及其受體(IL-17R)在鼻息肉和正常鼻黏膜中的表達及IL-17在鼻息肉患者血清中的表達,觀察IL-17 在鼻息肉發病過程中可能的作用機制和意義。方法 鼻息肉組織及血清標本來自青島大學附屬醫院耳鼻咽喉科2016年11月~2017年11月行鼻息肉切除術的42例患者,選取10例同期行上頜竇囊腫手術切除的正常中鼻道黏膜組織作為正常鼻粘膜對照,10例健康成年人血清作為正常對照。HE 染色用于常規組織病理學檢查,免疫組織化學染色觀察IL-17及其受體表達水平及部位,雙重免疫組織化學染色觀察表達IL-17的細胞,ELISA法檢測血清中IL-17蛋白表達水平。結果 ①42例鼻息肉組織中IL-17和IL-17R陽性細胞數較正常鼻黏膜組織中多,差異有統計學意義(P<0.05);巨噬細胞表面標志(CD68)在鼻息肉組織中的表達比正常鼻黏膜組織高,差異有統計學意義(P<0.05)。②IL-17及CD68雙重免疫組化染色顯示IL-17主要表達于鼻息肉巨噬細胞上,IL-17R主要表達于上皮層基底細胞、腺管基底細胞及血管內皮細胞。③42例鼻息肉患者血清中IL-17蛋白水平和健康成年人比較,差異無統計學意義(P>0.05)。結論 IL-17對鼻息肉的形成作用可能是通過影響巨噬細胞的功能及相關細胞因子實現的,IL-17與IL-17受體的相互作用可能參與鼻息肉的病理改變,如上皮細胞基底膜增厚及腺體增生等。
Abstract:
Objective To detect the expression of interleukin-17 (IL-17) and its receptor (IL-17R) in nasal polyps and normal nasal mucosa and the expression of IL-17 in serum of patients with nasal polyps, and to observe the incidence of IL-17 in nasal polyps. Possible mechanisms and implications of the process. Methods Nasal polyps and serum samples were obtained from 42 patients with ENT, from November 2016 to November 2017 in the Department of Otorhinolaryngology, Affiliated Hospital of Qingdao University. Ten normal nasal mucosa were removed from the maxillary sinus cyst. Tissue was used as a normal nasal mucosa control and 10 healthy adult sera were used as normal controls. HE staining was used for routine histopathological examination. The expression level and location of IL-17 and its receptor were observed by immunohistochemical staining. The cells expressing IL-17 were observed by double immunohistochemical staining. The serum IL-17 protein expression was detected by ELISA level.Results ①The number of IL-17 and IL-17R positive cells in 42 cases of nasal polyps was higher than that in normal nasal mucosa,the difference was statistically significant (P<0.05). The expression of macrophage surface marker (CD68) in nasal polyps was higher than that in normal nasal mucosa,the difference was statistically significant (P<0.05).②IL-17 and CD68 double immunohistochemical staining showed that IL-17 was mainly expressed on nasal polyp macrophages, and IL-17R was mainly expressed in epithelial basal cells, glandular basal cells and vascular endothelial cells.③There was no significant difference in serum IL-17 protein levels between 42 patients with nasal polyps and healthy adults (P>0.05). Conclusion The formation of IL-17 on nasal polyps may be achieved by affecting the function of macrophages and related cytokines. The interaction of IL-17 with IL-17 receptor may be involved in the pathological changes of nasal polyps membrane thickening and glandular hyperplasia.

參考文獻/References:

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更新日期/Last Update: 2019-11-15
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