[1]唐細田,肖 琳,徐彤慧.化學發光法和酶聯免疫法在乙型肝炎病毒血清學檢驗中的效果比較[J].醫學信息,2019,(22):164-165.[doi:10.3969/j.issn.1006-1959.2019.22.057]
 TANG Xi-tian,XIAO Lin,XU Tong-hui.Comparison of the Effects of Chemiluminescence and Enzyme-linked Immunosorbent Assay on Serological Test of Hepatitis B Virus[J].Medical Information,2019,(22):164-165.[doi:10.3969/j.issn.1006-1959.2019.22.057]
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化學發光法和酶聯免疫法在乙型肝炎病毒血清學檢驗中的效果比較()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2019年22期
頁碼:
164-165
欄目:
診療技術
出版日期:
2019-11-15

文章信息/Info

Title:
Comparison of the Effects of Chemiluminescence and Enzyme-linked Immunosorbent Assay on Serological Test of Hepatitis B Virus
文章編號:
1006-1959(2019)22-0164-02
作者:
唐細田肖 琳徐彤慧
(峽江縣人民醫院檢驗科,江西 峽江 331409)
Author(s):
TANG Xi-tianXIAO LinXU Tong-hui
(Department of Clinical Laboratory,Xiajiang County People's Hospital,Xiajiang 331409,Jiangxi,China)
關鍵詞:
酶聯免疫法化學發光法乙型肝炎病毒血清學檢驗
Keywords:
Enzyme-linked immunosorbent assayChemiluminescenceHepatitis B virusSerological test
分類號:
R512.62;R446.6
DOI:
10.3969/j.issn.1006-1959.2019.22.057
文獻標志碼:
A
摘要:
目的 比較化學發光法和酶聯免疫法在乙型肝炎病毒血清學檢驗中的效果。方法 選取2017年2月~2019年5月我院收治的疑似乙型肝炎患者63例作為研究對象,均予以酶聯免疫法、化學發光法進行血清學檢驗,以熒光定量PCR為金標準,比較兩種檢驗方式準確性、血清學指標檢出率。結果 共63例疑似乙肝患者,熒光定量PCR檢驗陽性38例、陰性25例;酶聯免疫法和化學發光法中準確度、靈敏度、特異度比較,差異無統計學意義(P>0.05)。38例乙肝陽性患者中,酶聯免疫法HBsAb、HBcAb陽性檢出率與化學發光法比較,差異無統計學意義(P>0.05);酶聯免疫法HBeAb、HBeAg、HBsAg陽性檢出率低于化學發光法,差異有統計學意義(P<0.05)。結論 乙型肝炎病毒血清學檢驗中,采用酶聯免疫法、化學發光法均具良好診斷效果,但在血清標志物檢驗中,化學發光法檢出效果更佳,有利于保證臨床診斷準確性。
Abstract:
Objective To compare the effects of chemiluminescence and enzyme-linked immunosorbent assay on the serological test of hepatitis B virus. Methods A total of 63 patients with suspected hepatitis B admitted to our hospital from February 2017 to May 2019 were selected as subjects.Evidence-linked immunosorbent assay and chemiluminescence assay were used for serological tests. Fluorescence quantitative PCR was used as the gold standard. The accuracy of the two test methods and the detection rate of serological markers were compared. Results A total of 63 patients with suspected hepatitis B were positive for fluorescent quantitative PCR and 38 for negative,there were 25 cases of negative; there was no significant difference in accuracy, sensitivity and specificity between enzyme-linked immunosorbent assay and chemiluminescence method (P>0.05). In 38 cases of hepatitis B positive patients, the positive detection rate of HBsAb and HBcAb by enzyme-linked immunosorbent assay was not significantly different from that of chemiluminescence method (P>0.05). The positive detection rate of HBeAb, HBeAg and HBsAg was lower than that of enzyme-linked immunosorbent assay. The chemiluminescence method was statistically significant (P<0.05). Conclusion In the serological test of hepatitis B virus, both enzyme-linked immunosorbent assay and chemiluminescence method have good diagnostic results. However, in the serum marker test, the chemiluminescence method has better detection effect, which is beneficial to ensure the accuracy of clinical diagnosis.

參考文獻/References:

[1]張輝,吳冬生,方敏,等.熒光定量PCR法與酶聯免疫吸附法在乙型肝炎病毒檢測中的應用[J].解放軍預防醫學雜志,2017,35(11):1459-1461. [2]高永慶.化學發光酶免疫分析法與酶聯免疫吸附法檢測乙肝病毒標志物比較[J].臨床軍醫雜志,2019,47(2):110-111. [3]許龍.化學發光法和酶聯免疫法檢測乙型肝炎病毒血清學的對比[J].中國醫藥指南,2018,16(27):68-69. [4]何成山,馬晨蕓,陸志成.化學發光免疫分析法檢測HBcAb陽性、HBsAg陰性血清中隱匿性乙型肝炎病毒感染的分析[J].標記免疫分析與臨床,2019,26(1):135-138,177.

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更新日期/Last Update: 2019-11-15
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